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Specifications
- Target protein:Leptin
- Description:LEPTIN-Sensitive-ELISA
- Size:1 kit
- Cat. No.:102994-280
- Supplier no.:E077
Specifications
About this item
Leptin Sens EIA measures human Leptin in human serum, plasma, urine, saliva and breast milk samples.
- Dynamic Range: 0.05 - 5 ng/ml
- Sensitivity: 0.01 ng/ml
- Incubation: 1.75 hours
Leptin, the product of the ob gene (1,2), is a recently discovered single-chain proteohormone with a molecular weight of 16 kD, which is thought to play a key role in the regulation of body weight. Its amino acid sequence exhibits no major homologies with other proteins (1). Leptin is almost exclusively produced by differentiated adipocytes (3-5). It acts on the central nervous system, in particular the hypothalamus, thereby suppressing food intake and stimulating energy expenditure (2,6-9). Leptin receptors - alternatively spliced forms exist that differ in length - belong to the cytokine class I receptor family (10-12). They are found ubiquitously in the body (10, 11, 13, 14) indicating a general role of leptin, which is currently not fully understood. A circulating form of the leptin receptor exists which acts as one of several leptin binding proteins (15). Besides its metabolic effects, leptin was shown to have a strong influence on a number of Endocrinology axes. In male mice, it blunted the starvation-induced marked decline of LH, testosterone, thyroxine and the increase of ACTH and corticosterone. In female mice, leptin prevented the starvation-induced delay in ovulation (16). Ob/ob mice, which are leptin deficient due to an ob gene mutation, are infertile. This defect could be corrected by administration of leptin, but not through weight loss due to fasting (17), suggesting that leptin is pivotal for reproductive functions.