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- Host:
- Primary antibody reactivity:
- Description:Anti tTg IgG (Tissue Transglutaminase) ELISA
- Size:1 kit
- Environmentally Preferable:
- Cat. No.:102995-022
- Supplier no.:TGG31-K01
Anti-hu tTG IgG ELISA determines IgG autoantibodies to tissue transglutaminase in serum or plasma.
- Dynamic Range: 1-300 U/mL
- Sensitivity: 1U/mL
- Incubation: 2.5 hours
Celiac disease, or gluten-sensitivity, is found already in neonates and is characterized by small intestinal damages leading to a so-called “flat” mucosa. Due to this extensive lesions mal-absorption occurs frequently accompanied with a depletion of key nutrients. Gliadin the alcohol soluble fraction of gluten represents the causative agent of celiac disease that provokes an inflammatory process in the small intestine. Gliadin is a substrate of tTG and cross-linked into high molecular complexes triggering probably both cellular and humoral immune responses. Incidence rates for celiac disease range from 1 in 300 (Western Ireland) to 1 in 4700 in European countries. However, a high number of subclinical cases of celiac disease have been detected by in-vitro tests revealing a prevalence of 4 in 1000. Individuals suffering from prolonged celiac disease additionally face an elevated risk of developing T cell lymphoma.Diagnosis of celiac disease comprises small intestine biopsy demonstrating a “flat” mucosa prior to a gluten-free diet and the following reconstitution of the mucosa after onset of the diet. Determination of anti-gliadin IgG and IgA by ELISA as well as the detection of anti-endomysium IgA by immunofluorescence has been considered as the main serological parameters for celiac disease so far.The identification of tissue transglutaminase as one of the main endomysial autoAntigenss and the availability of an easy to use and reliable ELISA kit employing recombinant human tissue transglutaminase pro¬mises the extension of diagnostic opportunities for celiac disease in future.IgA deficient celiac patients may demonstrate IgG autoantibodies to tTG only. Therefore, tTG IgG autoantibodies are the only serological parameter for celiac disease detectable in such patients apart from Gliadin IgG antibodies.