ESF AF™ insect cell culture medium is a complete serum-free, protein-free medium developed for robust cell growth, protein expression and baculovirus vector production for a wide range of insect cells.

• Animal component-free (100%)
• Comparable to ESF 921 medium for robust cell growth and expression
• Sf9 cell densities of 20×106 cells/ml or greater
• Ideal for scale-up manufacturing
• Works with a wide variety of baculovirus and host cell platforms
• Optimized for applications such as production of sub-unit vaccines, virus-like particles and gene therapy vectors (rAAV - all serotypes)
• Complete, ready-to-use, contains L-Glutamine
• Adapted cells available in suspension

ESF AF is an animal origin-free formulation for insect cell culture. It has been thoroughly tested on Sf9, Sf21, Tni (High Five™) and S2 cell lines and is comparable to ESF 921 for growth and expression. ESF AF is the logical choice for further manufacturing under cGMP/FDA regulatory compliance.