Alkali-Cation™ Yeast Transformation Kit is easy to use, robust, and its scalability makes this the method of choice for the high efficiency transformation needed in two hybrid screens.

• Transformation of yeast cells
• Components: Reagents: pH 7.5 TE, Lithium/Cesium Acetate, Carrier DNA 10 mg/mL, Sterile Solution Histamine, TE/Cation MIXX, Sterile Solution SOS.
• Store at Room Temperature (15-30 °C). With an exception for few reagents which need to be stored in following conditions: Sterile Carrier DNA (10 mg/mL): 4 °C, Sterile Solution Histamine: 4 °C.

Alkali-Cation™ Yeast Transformation Kit provides an efficient method for yeast transfomation and has been successfully used to transform linear DNA into Pichia pastoris for protein expression studies.

Alkali-Cation™ Yeast Transformation Kit provides all of the reagents required to perform an easy and quick non-electrical method for transforming yeast with linear or plasmid DNA. Host cells are made competent by treatment with a Lithium/Cesium Acetate mixture. The entire procedure may be completed in less than 90 minutes and routinely provides a transformation efficiency of greater than 104 transformants per µg of DNA.

Grow yeast to mid log phase, treat cells with Lithium/Cesium Acetate Solution, Introduce plasmid DNA, carrier DNA, histamine, PEG and TE/Cation MIXX, Heat shock and plate on regeneration media to allow cell wall reconstruction and growth of transformants.