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Anti-CD6 Mouse Monoclonal Antibody [clone: SPM547]
Anti-CD6 Mouse Monoclonal Antibody [clone: SPM547]
Catalog # 76195-372
Supplier:  Prosci
Anti-CD6 Mouse Monoclonal Antibody [clone: SPM547]
Catalog # 76195-372
Supplier:  Prosci
Supplier Number:  34-188

Specifications

  • Antibody Type:
    Primary
  • Antigen Name:
    CD6 molecule
  • Antigen Symbol:
    CD6
  • Clonality:
    Monoclonal
  • Clone:
    SPM547
  • Conjugation:
    Unconjugated
  • Flow Cytometry:
    Yes
  • Host:
    Mouse
  • ImmunoChemistry:
    Yes
  • ImmunoFluorescence:
    Yes
  • Isotype:
    IgG1
  • Reactivity:
    Human
  • Size:
    100 ug
  • Format:
    Purified Antibody
  • Form:
    Liquid
  • Gene ID:
    P30203
  • Antigen Synonyms:
    CD6
  • Storage Buffer:
    PBS with 0.1 mg/ml BSA and 0.05% sodium azide
  • Storage Temperature:
    Aliquot and Store at 2…8 °C. Avoid freez-thaw cycles.
  • Concentration:
    0.2 mg/mL
  • Shipping Temperature:
    4 °C
  • Immunogen:
    Human recombinant protein was used as the immunogen for this anti-CD6 antibody.
  • Purification:
    Protein G affinity chromatography
  • Cat. No.:
    76195-372

Specifications

About this item

CD6 is a type I transmembrane glycoprotein that contains a 24-amino acid signal sequence, three extracellular 'scavenger receptor cysteine-rich' (SRCR) domains, a membrane-spanning domain and a 44-amino acid cytoplasmic domain. The CD6 glycoprotein is tyrosine phosphorylated during TCR-mediated T cell activation. CD6 shows significant homology to CD5. CD6 is present on mature thymocytes, peripheral T cells and a subset of B cells. Antibodies to CD6 are used to deplete T cells from bone marrow transplants to prevent graft versus host disease.

Flow Cytometry: 0.5-1 ug/million cells in 0.1ml Immunofluorescence: 0.5-1 ug/ml Immunohistochemistry (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1) Prediluted format: incubate for 30 min at RT (2) The optimal dilution of the anti-CD6 antibody for each application should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Type: Primary
Antigen: CD6
Clonality: Monoclonal
Clone: SPM547
Conjugation: Unconjugated
Epitope:
Host: Mouse
Isotype: IgG1
Reactivity: Human