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Specifications
- Antibody Type:Primary
- Antigen Name:vimentin
- Antigen Symbol:VIM
- Clonality:Monoclonal
- Clone:SPM576
- Conjugation:Unconjugated
- Flow Cytometry:Yes
- Host:Mouse
- ImmunoChemistry:Yes
- ImmunoFluorescence:Yes
- Isotype:IgG1
- Reactivity:Pig,Goat,Chicken,Human,Bovine,Dog,Cat
- Western Blot:Yes
- Size:100 ug
- Format:Purified Antibody
- Form:Liquid
- Gene ID:P08670
- Antigen Synonyms:VIM
- Storage Buffer:PBS with 0.1 mg/ml BSA and 0.05% sodium azide
- Storage Temperature:Aliquot and Store at 2…8 °C. Avoid freez-thaw cycles.
- Concentration:0.2 mg/mL
- Shipping Temperature:4 °C
- Immunogen:Human recombinant protein was used as the immunogen for this VIM antibody.
- Purification:Protein G affinity chromatography
- Cat. No.:76195-360
Specifications
About this item
This mAb reacts with a 58kDa protein identified as vimentin. It shows no cross-reaction with other closely related intermediate filament proteins (IFPs) such as desmin, keratin, neurofilament, and glial fibrillary acid protein. Anti-vimentin alone is of limited value as a diagnostic tool; however, when used in panels with other antibodies, it is useful for the sub-classification of a given tumor. Expression of vimentin, when used in conjunction with anti-keratin, is helpful when distinguishing melanomas from undifferentiated carcinomas and large cell lymphomas. All melanomas and Schwannomas react strongly with anti-vimentin. It labels a variety of mesenchymal cells, including melanocytes, lymphocytes, endothelial cells, and fibroblasts. Non-reactivity of anti-vimentin is often considered more useful than its positive reactivity, since there are a few tumors that do not contain vimentin, e.g. hepatoma and seminoma. Anti-vimentin is also useful as a tissue process control reagent.
Flow Cytometry: 0.5-1 ug/million cells in 0.1ml Immunofluorescence: 1-2 ug/ml Western blot: 0.25-0.5 ug/ml Immunohistochemistry (FFPE): 0.25-0.5 ug/ml for 30 min at RT (1) Prediluted format: incubate for 30 min at RT (2) The optimal dilution of the VIM antibody for each application should be determined by the researcher.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Type: Primary
Antigen: VIM
Clonality: Monoclonal
Clone: SPM576
Conjugation: Unconjugated
Epitope:
Host: Mouse
Isotype: IgG1
Reactivity: Human, Bovine, Dog, Cat, Pig, Goat, Chicken