Cyclin D1 (CCND1), one of the key cell cycle regulators, is a putative proto-oncogene overexpressed in a wide variety of human neoplasms. About 60% of mantle cell lymphomas (MCL) contain a t(11; 14)(q13; q32) translocation resulting in over-expression of Cyclin D1. This antibody is useful in identifying mantle cell lymphomas (CCND1+) from CLL/SLL and follicular lymphomas (CCND1-). Occasionally, hairy cell leukemia and plasma cell myeloma weakly express CCND1.

Flow Cytometry: 0.5-1 ug/million cells in 0.1ml Immunofluorescence: 1-2 ug/ml Western blot: 0.5-1 ug/ml Immunohistochemistry (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1) Prediluted format: incubate for 30 min at RT (2) The optimal dilution of the CCND1 antibody for each application should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Type: Primary
Antigen: CCND1
Clonality: Monoclonal
Clone: SPM587
Conjugation: Unconjugated
Epitope:
Host: Mouse
Isotype: IgG2a, kappa
Reactivity: Human, Monkey, Mouse, Rat