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Anti-KLK3 Mouse Monoclonal Antibody [clone: SPM352]
Anti-KLK3 Mouse Monoclonal Antibody [clone: SPM352]
  76196-174
 :  Prosci
Anti-KLK3 Mouse Monoclonal Antibody [clone: SPM352]
  76196-174
 :  Prosci
 :  34-120

 

  • Antibody Type:
    Primary
  • Antigen Name:
    kallikrein-related peptidase 3
  • Antigen Symbol:
    KLK3
  • Clonality:
    Monoclonal
  • Clone:
    SPM352
  • Conjugation:
    Unconjugated
  • Flow Cytometry:
    Yes
  • Host:
    Mouse
  • ImmunoChemistry:
    Yes
  • ImmunoFluorescence:
    Yes
  • Isotype:
    IgG1 kappa
  • Reactivity:
    Human
  • Size:
    100 ug
  • Format:
    Purified Antibody
  • Form:
    Liquid
  • Gene ID:
    P07288
  • Antigen Synonyms:
    KLK3
  • Storage Buffer:
    PBS with 0.1 mg/ml BSA and 0.05% sodium azide
  • Storage Temperature:
    Aliquot and Store at 2…8 °C. Avoid freez-thaw cycles.
  • Concentration:
    0.2 mg/mL
  • Shipping Temperature:
    4 °C
  • Immunogen:
    Prostate specific antigen from human sperm plasma was used as the immunogen for this PSA antibody.
  • Purification:
    Protein G affinity chromatography
  • Cat. No.:
    76196-174

 

 

Recognizes a single protein of 33-34kDa, identified as the prostate specific antigen (PSA). This mAb is highly specific to PSA and stains prostatic secretory and ductal epithelium in both normal and neoplastic tissues. PSA is a chymotrypsin-like serine protease (kallikrein family) exclusively produced by the prostate epithelium, and abundant in seminal fluid. PSA can be detected in the sera of patients with prostatic carcinoma. It is predominantly complexed to a liver-derived serine protease inhibitor, alpha-1-antichymotrypsin (ACT). A higher proportion of serum PSA is complexed to ACT in prostate cancer than in benign prostate hyperplasia.

Flow Cytometry: 0.5-1 ug/million cells in 0.1ml Immunofluorescence: 0.5-1 ug/ml Immunohistochemistry (FFPE): 0.25-0.5 ug/ml for 30 min at RT (1) Prediluted format: incubate for 30 min at RT (2) The optimal dilution of the PSA antibody for each application should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Type: Primary
Antigen: KLK3
Clonality: Monoclonal
Clone: SPM352
Conjugation: Unconjugated
Epitope:
Host: Mouse
Isotype: IgG1, kappa
Reactivity: Human