MART-1/Melan-A is a protein antigen that is found on the surface of melanocytes. Antibodies against the antigen are used in the medical specialty of anatomic pathology in order to recognize cells of melanocytic differentiation, useful for the diagnosis of a melanoma. The MART-1/Melan-A antigen is specific for the melanocyte lineage, found in normal skin, the retina, and melanocytes, but not in other normal tissues. It is thus useful as a marker for melanocytic tumors (melanomas) with the caveat that it is normally found in benign nevi as well. [Wiki]

Western blot: 0.5-1 ug/ml Flow Cytometry: 0.5-1ug/10^6 cells Immunofluorescence: 0.5-1 ug/ml Immunohistochemistry (FFPE): 0.5-1 ug/ml for 30 minutes at RT Prediluted IHC only format: incubate for 30 min at RT (1) The optimal dilution of the recombinant Melan-A antibody for each application should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Type: Primary
Antigen: MLANA
Clonality: Monoclonal
Clone: MLANA/1761R
Conjugation: Unconjugated
Epitope:
Host: Rabbit
Isotype: IgG, k
Reactivity: Human, Mouse, Rat