Friend leukemia integration 1 is a member of the ETS family of DNA binding transcription factors that is involved in cellular proliferation and tumorigenesis. Members of the Ets gene family share a highly conserved carboxy-terminal domain containing a sequence related to the SV40 large T antigen nuclear localization signal sequence. Approximately 90% of Ewing's Sarcoma (EWS) / Primitive Neuroectodermal Tumors (PNET) have a specific translocation, t(11;22)(q24;q12), which results in fusion of EWS to Fli-1, and production of an EWS-Fli-1 fusion protein. Among normal tissues only endothelial cells and small lymphocytes express Fli-1. This protein is expressed in majority of vascular tumors including angiosarcomas, hemangioendotheliomas, hemangiomas, and Kaposi's Sarcomas. High sensitivity and specificity of Fli-1 equals to or exceeds that of the established vascular markers like CD31, CD34, and Factor VIII.

Western blot: 1-2 ug/m;l Flow Cytometry: 0.5-1ug/10^6 cells IF: 1-2 ug/ml The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the FLI1 antibody to be titered up or down for optimal performance.

Type: Primary
Antigen: FLI1
Clonality: Monoclonal
Clone: FLVI1-1
Conjugation: Unconjugated
Epitope:
Host: Mouse
Isotype: IgG
Reactivity: Human