Specifications
- Antibody Type:Primary
- Antigen Name:intercellular adhesion molecule 3
- Antigen Symbol:ICAM3
- Clonality:Monoclonal
- Clone:CDLA50-1
- Conjugation:Unconjugated
- Flow Cytometry:Yes
- Host:Mouse
- ImmunoChemistry:Yes
- ImmunoFluorescence:Yes
- Isotype:IgG2b kappa
- Reactivity:Human
- Western Blot:Yes
- Size:100 ug
- Format:Purified Antibody
- Form:Liquid
- Gene ID:P32942
- Antigen Synonyms:ICAM3
- Storage Buffer:PBS with 0.1 mg/ml BSA and 0.05% sodium azide
- Storage Temperature:Aliquot and Store at 2…8 °C. Avoid freez-thaw cycles.
- Concentration:0.2 mg/mL
- Shipping Temperature:4 °C
- Immunogen:Recombinant human protein was used as the immunogen for the ICAM3 antibody.
- Purification:Protein G affinity chromatography
- Cat. No.:76194-986
Specifications
About this item
ICAM3/CD50 is a member of the intercellular adhesion molecule (ICAM) family. All ICAM proteins are type I transmembrane glycoproteins, contain 2-9 immunoglobulin-like C2-type domains, and bind to the leukocyte adhesion LFA-1 protein. This protein is constitutively and abundantly expressed by all leucocytes and may be the most important ligand for LFA-1 in the initiation of the immune response. It functions not only as an adhesion molecule, but also as a potent signalling molecule. [RefSeq]
Flow Cytometry: 0.5-1 ug/million cells in 0.1ml Immunofluorescence: 1-2 ug/ml Western blot: 1-2 ug/ml Immunohistochemistry (FFPE): 1-2 ug/ml for 30 min at RT (1) Prediluted format : incubate for 30 min at RT (2) Optimal dilution of the ICAM3 antibody should be determined by the researcher.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Type: Primary
Antigen: ICAM3
Clonality: Monoclonal
Clone: CDLA50-1
Conjugation: Unconjugated
Epitope:
Host: Mouse
Isotype: IgG2b, kappa
Reactivity: Human