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Puerto Rico
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Anti-TYMS Mouse Monoclonal Antibody [clone: TYSY9]
  76196-076
 :  Prosci
Anti-TYMS Mouse Monoclonal Antibody [clone: TYSY9]
  76196-076
 :  Prosci
 :  33-918

 

  • Antibody Type:
    Primary
  • Antigen Name:
    thymidylate synthetase
  • Antigen Symbol:
    TYMS
  • Clonality:
    Monoclonal
  • Clone:
    TYSY9
  • Conjugation:
    Unconjugated
  • Host:
    Mouse
  • ImmunoChemistry:
    Yes
  • ImmunoFluorescence:
    Yes
  • Isotype:
    IgG1 kappa
  • Reactivity:
    Human
  • Size:
    100 ug
  • Format:
    Purified Antibody
  • Form:
    Liquid
  • Gene ID:
    P04818
  • Antigen Synonyms:
    TYMS
  • Storage Buffer:
    PBS with 0.1 mg/ml BSA and 0.05% sodium azide
  • Storage Temperature:
    Aliquot and Store at 2…8 °C. Avoid freez-thaw cycles.
  • Concentration:
    0.2 mg/mL
  • Shipping Temperature:
    4 °C
  • Immunogen:
    Recombinant human protein was used as the immunogen for this Thymidylate synthase antibody.
  • Purification:
    Protein G purified monoclonal antibody
  • Cat. No.:
    76196-076

 

 

Thymidylate synthase converts deoxyuridine monophosphate (dUMP) to deoxythymidine monophosphate (dTMP), which is essential for DNA biosynthesis. Thymidylate synthase is also a critical target for the fluoropyrimidines, an important group of anti-neoplastic drugs that are widely used in the treatment of solid tumors. Both 5-FU and fluorodeoxyuridine are converted in tumor cells to FdUMP which inactivates thymidylate synthase by formation of a ternary covalent complex in the presence of the folate cofactor 5,10-methylenetetrahydrofolate. Expression of thymidylate synthase protein is associated with response to 5-fluorouracil (5-FU) in human colorectal, gastric, head and neck, and breast carcinomas.

Immunofluorescence: 0.5-1 ug/ml IHC (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1) Prediluted format : incubate for 30 min at RT (2) The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Thymidylate synthase antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Type: Primary
Antigen: TYMS
Clonality: Monoclonal
Clone: TYSY9
Conjugation: Unconjugated
Epitope:
Host: Mouse
Isotype: IgG1, kappa
Reactivity: Human