- Antibody Type:Primary
- Antigen Name:NK2 homeobox 2
- Antigen Symbol:NKX2-2
- Clonality:Monoclonal
- Clone:NOX4/1245
- Conjugation:Unconjugated
- ELISA:Yes
- Flow Cytometry:Yes
- Host:Mouse
- ImmunoFluorescence:Yes
- Isotype:IgG2b kappa
- Reactivity:Human
- Western Blot:Yes
- Size:100 ug
- Format:Purified Antibody
- Form:Liquid
- Gene ID:Q9NPH5
- Antigen Synonyms:NKX2-2
- Storage Buffer:PBS with 0.1 mg/ml BSA and 0.05% sodium azide
- Storage Temperature:Aliquot and Store at 2…8 °C. Avoid freez-thaw cycles.
- Concentration:0.2 mg/mL
- Shipping Temperature:4 °C
- Immunogen:Human recombinant protein was used as the immunogen for this NOX4 antibody.
- Purification:Protein G affinity chromatography
- Cat. No.:76194-676
The superoxide-generating NADPH oxidase includes a membrane-bound flavocytochrome containing two subunits, gp91-phox and p22-phox, and the cytosolic proteins p47-phox and p67-phox. During activation of the NADPH oxidase, p47-phox and p67-phox migrate to the plasma membrane where they associate with the flavocytochrome, cytochrome b558, to form the active enzyme complex. The p22 and gp91-phox subunits also function as surface O2 sensors that initiate cellular signaling in response to hypoxic conditions. NOX4 is a renal gp91-phox homolog highly expressed at the site of erythropoietin production in the proximal convoluted tubule epithelial cells of the renal cortex. It is also expressed in fetal tissues, placenta, glioblastoma and vascular cells.
Flow Cytometry: 0.5-1ug/10^6 cells in 0.1ml IF: 1-2 ug/ml ELISA: suitable; order BSA-free formulation for coating WB: 0.5-1 ug/ml Titering of the NOX4 antibody may be required for optimal performance.
Type: Primary
Antigen: NKX2-2
Clonality: Monoclonal
Clone: NOX4/1245
Conjugation: Unconjugated
Epitope:
Host: Mouse
Isotype: IgG2b, kappa
Reactivity: Human