Specifications
- Antibody Type:Primary
- Antigen Name:protein tyrosine phosphatase, receptor type, C
- Antigen Symbol:PTPRC
- Clonality:Monoclonal
- Clone:PTPRC/1461
- Conjugation:Unconjugated
- Flow Cytometry:Yes
- Host:Mouse
- ImmunoChemistry:Yes
- ImmunoFluorescence:Yes
- Isotype:IgG2a kappa
- Reactivity:Human
- Western Blot:Yes
- Size:100 ug
- Format:Purified Antibody
- Form:Liquid
- Gene ID:P08575
- Antigen Synonyms:PTPRC
- Storage Buffer:PBS with 0.1 mg/ml BSA and 0.05% sodium azide
- Storage Temperature:Aliquot and Store at 2…8 °C. Avoid freez-thaw cycles.
- Concentration:0.2 mg/mL
- Shipping Temperature:4 °C
- Immunogen:Human B lymphocytes were used as the immunogen for the CD45 antibody.
- Purification:Protein G affinity chromatography
- Cat. No.:76194-530
Specifications
About this item
CD45R, also designated CD45 and PTPRC, has been identified as a transmembrane glycoprotein, broadly expressed among hematopoietic cells. Multiple isoforms of CD45R are distributed throughout the immune system according to cell type. These isoforms arise because of alternative splicing of exons 4, 5, and 6. The corresponding protein domains are characterized by the binding of monoclonal antibodies specific for CD45RA (exon 4), CD45RB (exon 5), CD45RC (exon 6) and CD45RO (exons 4 to 6 spliced out). The variation in these isoforms is localized to the extracellular domain of CD45R, while the intracellular domain is conserved. CD45R functions as a phosphor-tyrosine phosphatase. Antibody to CD45 is useful in differential diagnosis of lymphoid tumors from non-hematopoietic undifferentiated neoplasms.
Flow Cytometry: 0.5-1 ug/million cells Immunofluorescence: 0.5-1 ug/ml Immunohistology (FFPE): 0.5-1.0 ug/ml for 30 minutes at RT Due to variation in protocol and secondary antibody used, the CD45 antibody may need to be titered for optimal performance.
Type: Primary
Antigen: PTPRC
Clonality: Monoclonal
Clone: PTPRC/1461
Conjugation: Unconjugated
Epitope:
Host: Mouse
Isotype: IgG2a, kappa
Reactivity: Human