- Antibody Type:Primary
- Antigen Name:transforming growth factor, alpha
- Antigen Symbol:TGFA
- Clonality:Monoclonal
- Clone:TG86
- Conjugation:Unconjugated
- Flow Cytometry:Yes
- Host:Mouse
- ImmunoChemistry:Yes
- ImmunoFluorescence:Yes
- Isotype:IgG1 kappa
- Reactivity:Zebrafish,Rabbit,Human
- Size:100 ug
- Format:Purified Antibody
- Form:Liquid
- Gene ID:P01135
- Antigen Synonyms:TGFA
- Storage Buffer:PBS with 0.1 mg/ml BSA and 0.05% sodium azide
- Storage Temperature:Aliquot and Store at 2…8 °C. Avoid freez-thaw cycles.
- Concentration:0.2 mg/mL
- Shipping Temperature:4 °C
- Immunogen:Amino acids 34-43 (PPVAAAVVSH) from the human protein were used as the immunogen for the TGFa antibody.
- Purification:Protein G affinity chromatography
- Cat. No.:76194-212
This antibody reacts with the TGF alpha and shows no cross-reaction with EGF and the neuropeptide synenkephalin. The staining with this mAb is completely blocked by the peptide used for raising this antibody. TGFa is a growth factor with 33% homology to EGF, binds to EGFR, activates tyrosine phosphorylation of the receptor, and stimulates cell proliferation. It plays a role in tumor initiation by inducing the reversible transformed phenotype.
Flow Cytometry: 0.5-1 ug/million cells in 0.1ml Immunofluorescence: 1-2 ug/ml Immunohistochemistry (FFPE): 2-4 ug/ml for 30 min at RT (1) Optimal dilution of the TGFa antibody should be determined by the researcher.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
Type: Primary
Antigen: TGFA
Clonality: Monoclonal
Clone: TG86
Conjugation: Unconjugated
Epitope:
Host: Mouse
Isotype: IgG1, kappa
Reactivity: Human, Rabbit, Zebrafish