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Specifications
- Antibody Type:Primary
- Antigen Name:heparan sulfate proteoglycan 2
- Antigen Symbol:HSPG2
- Clonality:Monoclonal
- Clone:A7L6
- Conjugation:Unconjugated
- Flow Cytometry:Yes
- Host:Rat
- ImmunoChemistry:Yes
- ImmunoFluorescence:Yes
- Isotype:IgG2a kappa
- Reactivity:Fish,Pig,Monkey,Human,Bovine,Mouse
- Western Blot:Yes
- Size:100 ug
- Format:Purified Antibody
- Form:Liquid
- Gene ID:P98160
- Antigen Synonyms:HSPG2
- Storage Buffer:PBS with 0.1 mg/ml BSA and 0.05% sodium azide
- Storage Temperature:Aliquot and Store at 2…8 °C. Avoid freez-thaw cycles.
- Concentration:0.2 mg/mL
- Shipping Temperature:4 °C
- Immunogen:A murine EHS laminin preparation was used as the immunogen for the Perlecan antibody.
- Purification:Protein G affinity chromatography
- Cat. No.:76194-114
Specifications
About this item
This mAb specifically precipitates heterogeneous material of high MW, identified as perlecan, a major heparan-sulfate proteoglycan (HSPG) within all basement membranes and cell surfaces. It does not cross-react with laminin, fibronectin, or dermatran sulfate proteoglycan. Because of perlecan s strategic location and ability to store and protect growth factors, it has been strongly implicated in the control of tumor cell growth and metastatic behavior. Perlecan possesses angiogenic and growth-promoting attributes primarily by acting as a co-receptor for basic fibroblast growth factor (FGF-2). Suppression of perlecan causes substantial inhibition of neoplastic growth and neovascularization. Thus, perlecan is a potent inducer of neoplasm growth and angiogenesis in vivo and therapeutic interventions targeting this key modulator of tumor progression may improve neoplastic treatment.
Flow Cytometry: 0.5-1 ug/million cells in 0.1ml Immunofluorescence: 0.5-1 ug/ml Immunohistochemistry (FFPE): 1-2 ug/ml for 30 min at RT (1) Prediluted format : incubate for 30 min at RT (2) Optimal dilution of the Perlecan antibody should be determined by the researcher.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Type: Primary
Antigen: HSPG2
Clonality: Monoclonal
Clone: A7L6
Conjugation: Unconjugated
Epitope:
Host: Rat
Isotype: IgG2a, kappa
Reactivity: Human, Monkey, Bovine, Pig, Mouse, Fish