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Anti-TP63 Rabbit Polyclonal Antibody
Anti-TP63 Rabbit Polyclonal Antibody
Catalog # 76195-674
Supplier:  Prosci
Anti-TP63 Rabbit Polyclonal Antibody
Catalog # 76195-674
Supplier:  Prosci
Supplier Number:  33-230

Specifications

  • Antibody Type:
    Primary
  • Antigen Name:
    tumor protein p63
  • Antigen Symbol:
    TP63
  • Clonality:
    Polyclonal
  • Conjugation:
    Unconjugated
  • Flow Cytometry:
    Yes
  • Host:
    Rabbit
  • ImmunoChemistry:
    Yes
  • ImmunoFluorescence:
    Yes
  • Isotype:
    IgG
  • Reactivity:
    Human,
    Bovine,
    Rat,
    Mouse
  • Western Blot:
    Yes
  • Size:
    100 ug
  • Format:
    Purified Antibody
  • Form:
    Liquid
  • Gene ID:
    Q9H3D4
  • Antigen Synonyms:
    TP63
  • Storage Buffer:
    PBS with 0.1 mg/ml BSA and 0.05% sodium azide
  • Storage Temperature:
    Aliquot and Store at 2…8 °C. Avoid freez-thaw cycles.
  • Concentration:
    0.2 mg/mL
  • Shipping Temperature:
    4 °C
  • Immunogen:
    Amino acids ENNAQTQFSEPQY (aa 5-17) of human p40 / p63 delta were used as the immunogen for this antibody.
  • Purification:
    Protein A affinity chromatography
  • Cat. No.:
    76195-674

Specifications

About this item

p40 (p63 delta) is a marker recently determined to be highly specific for squamous basal cells in the immunohistochemistry (IHC) application. The current more routinely recommended marker, p63, appears to have less specificity compared to p40, especially on squamous cell tumors. The ability to differentiate between lung adenocarcinoma vs. squamous cell carcinoma is difficult and has bearing on the different therapeutic avenues for each subtype treatment. p40 antibody’s ability to distinguish between the tumor types appears to be superior when compared to p63. The ability to utilize a p40 antibody probe as a squamous cell marker bolsters its use for future sub-classification of lung cancers, especially by immunohistochemical techniques.

Flow Cytometry: 0.5-1ug/10e6 cells IF: 1-2 ug/ml WB: 0.5-1 ug/ml IHC (FFPE): 1-2 ug/ml for 30 minutes at RT (1) Prediluted format : incubate for 30 min at RT (2) The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the p40 antibody to be titered up or down for optimal performance.

1. Staining of FFPE tissues requires boiling sections in 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Type: Primary
Antigen: TP63
Clonality: Polyclonal
Clone:
Conjugation: Unconjugated
Epitope:
Host: Rabbit
Isotype: IgG
Reactivity: Human, Mouse, Rat, Bovine