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Specifications
- Antibody Type:Primary
- Antigen Name:tumor protein p53
- Antigen Symbol:TP53
- Clonality:Monoclonal
- Clone:BP53-12
- Conjugation:Unconjugated
- Flow Cytometry:Yes
- Host:Mouse
- ImmunoChemistry:Yes
- ImmunoFluorescence:Yes
- Isotype:IgG2a
- Reactivity:Human
- Western Blot:Yes
- Size:100 ug
- Environmentally Preferable:
- Format:Purified Antibody
- Form:Liquid
- Gene ID:P04637
- Antigen Synonyms:TP53
- Storage Buffer:PBS with 0.1 mg/ml BSA and 0.05% sodium azide
- Storage Temperature:Aliquot and Store at 2…8 °C. Avoid freez-thaw cycles.
- Concentration:0.2 mg/mL
- Shipping Temperature:4 °C
- Immunogen:Recombinant human wild-type p53 protein was used as the immunogen for this antibody.
- Purification:Protein G affinity chromatography
- Cat. No.:76195-648
Specifications
About this item
This antibody is specific for a 53kDa protein, which is identified as p53 suppressor gene product. It reacts with the mutant as well as the wild form of p53 under denaturing and non-denaturing conditions. The antibody epitope maps within the N-terminus (aa 20-25) of p53 oncoprotein. p53 is a tumor suppressor gene expressed in a wide variety of tissue types and is involved in regulating cell growth, replication, and apoptosis. It binds to MDM2, SV40 T antigen and human papilloma virus E6 protein. Positive nuclear staining with p53 antibody has been reported to be a negative prognostic factor in breast carcinoma, lung carcinoma, colorectal, and urothelial carcinoma. antibody to p53 positivity has also been used to differentiate uterine serous carcinoma from endometrioid carcinoma as well as to detect intratubular germ cell neoplasia. Mutations involving p53 are found in a wide variety of malignant tumors, including breast, ovarian, bladder, colon, lung, and melanoma.
Flow Cytometry: 0.5-1 ug/million cells IF: 1-2 ug/ml WB: 0.5-1 ug/ml IHC (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1) Prediluted format : incubate for 30 min at RT (2) The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Type: Primary
Antigen: TP53
Clonality: Monoclonal
Clone: BP53-12
Conjugation: Unconjugated
Epitope:
Host: Mouse
Isotype: IgG2a
Reactivity: Human