- Antibody Type:Primary
- Antigen Name:NK2 homeobox 2
- Antigen Symbol:NKX2-2
- Clonality:Monoclonal
- Clone:NX2/294
- Conjugation:Unconjugated
- Flow Cytometry:Yes
- Host:Mouse
- ImmunoChemistry:Yes
- ImmunoFluorescence:Yes
- Isotype:IgG2b kappa
- Reactivity:Chicken,Human,Rat,Mouse
- Size:100 ug
- Format:Purified Antibody
- Form:Liquid
- Gene ID:O95096
- Antigen Synonyms:NKX2-2
- Storage Buffer:PBS with 0.1 mg/ml BSA and 0.05% sodium azide
- Storage Temperature:Aliquot and Store at 2…8 °C. Avoid freez-thaw cycles.
- Concentration:0.2 mg/mL
- Shipping Temperature:4 °C
- Immunogen:Human NKX2.2 recombinant protein was used as the immunogen for this antibody.
- Purification:Protein G affinity chromatography
- Cat. No.:76195-584
Expression of NKX2.2 has been found in neuroendocrine tumors of the gut, making it a potential marker for the study of gastrointestinal neuroendocrine tumors. More recently, NKX2.2 protein was identified as a target of EWS-FLI-1, the fusion protein specific to Ewing sarcoma, and was shown to be differentially upregulated in Ewing sarcoma on the basis of array-based gene expression analysis. antibody to NKX2.2 detects a valuable marker for Ewing sarcoma, with a sensitivity of 93% and a specificity of 89%, and aids in the differential diagnosis of small round cell tumors.
Flow Cytometry: 0.5-1 ug/million cells IF: 1-2 ug/ml IHC (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1) Prediluted format : incubate for 30 min at RT (2) The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the NKX2.2 antibody to be titered up or down for optimal performance.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Type: Primary
Antigen: NKX2-2
Clonality: Monoclonal
Clone: NX2/294
Conjugation: Unconjugated
Epitope:
Host: Mouse
Isotype: IgG2b, kappa
Reactivity: Human, Mouse, Rat, Chicken