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Anti-NKX2-2 Mouse Monoclonal Antibody [clone: NX2/294]
Anti-NKX2-2 Mouse Monoclonal Antibody [clone: NX2/294]
  76195-584
 :  Prosci
Anti-NKX2-2 Mouse Monoclonal Antibody [clone: NX2/294]
  76195-584
 :  Prosci
 :  33-181

 

  • Antibody Type:
    Primary
  • Antigen Name:
    NK2 homeobox 2
  • Antigen Symbol:
    NKX2-2
  • Clonality:
    Monoclonal
  • Clone:
    NX2/294
  • Conjugation:
    Unconjugated
  • Flow Cytometry:
    Yes
  • Host:
    Mouse
  • ImmunoChemistry:
    Yes
  • ImmunoFluorescence:
    Yes
  • Isotype:
    IgG2b kappa
  • Reactivity:
    Chicken,
    Human,
    Rat,
    Mouse
  • Size:
    100 ug
  • Format:
    Purified Antibody
  • Form:
    Liquid
  • Gene ID:
    O95096
  • Antigen Synonyms:
    NKX2-2
  • Storage Buffer:
    PBS with 0.1 mg/ml BSA and 0.05% sodium azide
  • Storage Temperature:
    Aliquot and Store at 2…8 °C. Avoid freez-thaw cycles.
  • Concentration:
    0.2 mg/mL
  • Shipping Temperature:
    4 °C
  • Immunogen:
    Human NKX2.2 recombinant protein was used as the immunogen for this antibody.
  • Purification:
    Protein G affinity chromatography
  • Cat. No.:
    76195-584

 

 

Expression of NKX2.2 has been found in neuroendocrine tumors of the gut, making it a potential marker for the study of gastrointestinal neuroendocrine tumors. More recently, NKX2.2 protein was identified as a target of EWS-FLI-1, the fusion protein specific to Ewing sarcoma, and was shown to be differentially upregulated in Ewing sarcoma on the basis of array-based gene expression analysis. antibody to NKX2.2 detects a valuable marker for Ewing sarcoma, with a sensitivity of 93% and a specificity of 89%, and aids in the differential diagnosis of small round cell tumors.

Flow Cytometry: 0.5-1 ug/million cells IF: 1-2 ug/ml IHC (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1) Prediluted format : incubate for 30 min at RT (2) The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the NKX2.2 antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Type: Primary
Antigen: NKX2-2
Clonality: Monoclonal
Clone: NX2/294
Conjugation: Unconjugated
Epitope:
Host: Mouse
Isotype: IgG2b, kappa
Reactivity: Human, Mouse, Rat, Chicken