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Anti-EGF R Mouse Monoclonal Antibody [clone: GFR450]
Anti-EGF R Mouse Monoclonal Antibody [clone: GFR450]
Catalog # 76195-500
Supplier:  Prosci
Anti-EGF R Mouse Monoclonal Antibody [clone: GFR450]
Catalog # 76195-500
Supplier:  Prosci
Supplier Number:  33-119

Specifications

  • Antibody Type:
    Primary
  • Antigen Name:
    epidermal growth factor receptor
  • Antigen Symbol:
    EGF R
  • Clonality:
    Monoclonal
  • Clone:
    GFR450
  • Conjugation:
    Unconjugated
  • Flow Cytometry:
    Yes
  • Host:
    Mouse
  • ImmunoChemistry:
    Yes
  • ImmunoFluorescence:
    Yes
  • Isotype:
    IgG2a kappa
  • Reactivity:
    Human
  • Western Blot:
    Yes
  • Size:
    100 ug
  • Format:
    Purified Antibody
  • Form:
    Liquid
  • Gene ID:
    P00533
  • Antigen Synonyms:
    EGF R
  • Storage Buffer:
    PBS with 0.1 mg/ml BSA and 0.05% sodium azide
  • Storage Temperature:
    Aliquot and Store at 2…8 °C. Avoid freez-thaw cycles.
  • Concentration:
    0.2 mg/mL
  • Shipping Temperature:
    4 °C
  • Immunogen:
    The immunizing protein for this antibody was recombinant human EGFR.
  • Purification:
    Protein G affinity chromatography
  • Cat. No.:
    76195-500

Specifications

About this item

This antibody recognizes a protein of 170kDa, identified as EGFR. EGFR is type I receptor tyrosine kinase with sequence homology to ErbB2, -3 -4 or HER2, -3 -4. It binds to Epidermal Growth Factor (EGF), Transforming Growth Factor-a (TGF-a), Heparin-binding EGF (HB-EGF), amphiregulin, betacellulin and epiregulin. EGFR is overexpressed in tumors of breast, brain, bladder, lung, gastric, head/neck, esophagus, cervix, vulva, ovary, and endometrium. It is predominantly present in squamous cell carcinomas.

IHC (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1) Flow Cytometry: 0.5-1 ug/million cells in 0.1ml IF: 1-2 ug/ml The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the EGFR antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.

Type: Primary
Antigen: EGF R
Clonality: Monoclonal
Clone: GFR450
Conjugation: Unconjugated
Epitope:
Host: Mouse
Isotype: IgG2a, kappa
Reactivity: Human