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- Antibody Type:Primary
- Antigen Name:activating transcription factor 6
- Antigen Symbol:ATF6
- Clonality:Monoclonal
- Clone:AF6229
- Conjugation:Unconjugated
- Host:Mouse
- ImmunoChemistry:Yes
- Isotype:IgG1 kappa
- Reactivity:Human,Rat,Mouse
- Western Blot:Yes
- Size:100 ug
- Environmentally Preferable:
- Format:Purified Antibody
- Form:Liquid
- Gene ID:P18850
- Antigen Synonyms:ATF6
- Storage Buffer:PBS with 0.1 mg/ml BSA and 0.05% sodium azide
- Storage Temperature:Aliquot and Store at –20 °C. Avoid freez-thaw cycles.
- Concentration:0.5 mg/mL
- Shipping Temperature:4 °C
- Immunogen:A recombinant protein fragment from the N-terminal region of human ATF6 was used as the immunogen for this antibody.
- Purification:Protein G purified monoclonal antibody
- Cat. No.:76194-026
ER stress can cause the accumulation of unfolded proteins and lead to the activation of the UPR (unfolded proetin response). Cells have developed an intracellular signaling pathway to deal with this stress, and Activating Transcription Factor 6 (ATF6) is a major transducer of the UPR. Upon activation, ATF6 seperates from GPR78 and is cleaved. The ~50 kD cytosolic portion of ATF6 translocates to the nucleus, transmitting the ER stress signal. In the nucleus, ATF6 activates the transcription of UPR-responsive genes by biniding to their ERSE (ER stress response element) region.
Western blot: 2-4 ug/ml (2) IHC (Paraffin): 5 ug/ml (1) ATF6 is a 670 amino acid protein that migrates in western blot between 70~90 kD due to glycosylation.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. ATF6 is seen in western blotting between 75~90KD.
Type: Primary
Antigen: ATF6
Clonality: Monoclonal
Clone: AF6229
Conjugation: Unconjugated
Epitope:
Host: Mouse
Isotype: IgG1, kappa
Reactivity: Human, Mouse, Rat