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Specifications
- Size:100 Assays
- Cat. No.:103010-670
- Storage temperature:– 20 °C
- Includes:The assays can be performed in a convenient 96-well microplate format, kit includes essential components and detailed protocol and references
- Supplier no.:AS-72227
Specifications
About this item
Tobacco Etch Virus (TEV) proteinase is widely used to remove fusion tags from recombinant proteins
The SensoLyte® TEV Protease Assay Kit uses TEV specific Glu-Asn-Leu-Tyr-Phe-Gln-Gly substrate labeled with 5-FAM dye and QXL® 520 quencher. Proteolytic cleavage of this quenched 5-FAM-QXL® 520 conjugate yields a brightly green fluorescence, which can be continuously monitored at Ex/Em= 490/520 nm. Increase in fluorescence intensity is directly proportional to the TEV protease quantity/activity.
It recognizes specific sequence Glu-Asn-Leu-Tyr-Phe-Gln-Gly and cleaves between Glutamine and Glycine residues. TEV protease is active even at 4 °C, making it the protease of choice for fusion proteins or tags removal.