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SensoLyte® Green SIRT2 Assay Kit (Fluorimetric), AnaSpec Inc.
SensoLyte® Green SIRT2 Assay Kit (Fluorimetric), AnaSpec Inc.
Catalog # 103010-586
Supplier:  Anaspec Inc
CAS Number:  
SensoLyte® Green SIRT2 Assay Kit (Fluorimetric), AnaSpec Inc.
Catalog # 103010-586
Supplier:  Anaspec Inc
Supplier Number:  AS-72188
CAS Number:  

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Specifications

  • Size:
    100 Assays
  • Cat. No.:
    103010-586
  • Storage temperature:
    – 78 °C
  • Includes:
    The assays can be performed in a convenient 96-well microplate format, kit includes essential components and detailed protocol and references
  • Supplier no.:
    AS-72188

Specifications

About this item

Histone deacetylase (HDAC) enzymes act as transcriptional repressors of genes through the deacetylation of lysine residues on histone proteins

The SensoLyte® Green SIRT2 Assay Kit provides a convenient, two-step homogeneous procedure for measuring sirtuin 2 activity using a fluorogenic substrate. In the first step, an acetylated substrate is incubated with SIRT2 containing samples. Deacetylation of substrate first sensitizes it, so that, in the second step, mixing with the SIRT developer generates a fluorophore, which can then be detected at Ex/Em= 490 nm/520 nm. This kit can be used for high throughput screening (HTS) of SIRT2 inhibitors/activators. The long wavelength fluorescence of SIRT2 substrate is less interfered by the autofluorescence of cell components and test compounds. Assays are performed in a standard 96-well plate format.

Sirtuins (SIRTs) are a unique class of deacetylases (class III HDACs) that require NAD+ as a cofactor. Sirtuin 2 (SIRT2) is a cytoplasmic protein involved in regulation of cell cycle, DNA repair and tumorogenesis.