Specifications
- Antibody Type:Primary
- Antigen Name:Heat shock 70kDa protein 8
- Antigen Symbol:HSPA8
- Clonality:Polyclonal
- Host:Rabbit
- ImmunoChemistry:Yes
- Isotype:IgG
- Reactivity:Human,Rat,Mouse
- Western Blot:Yes
- Size:100 µg/vial
- Form:Lyophilized
- Gene ID:P11142
- Antigen Synonyms:HSP73|HEL-S-72p|HSC70|LAP-1|HSC71|NIP71|HSPA10|HEL-33|HSC54|HSP71|LAP1
- UniProtKB:P11142
- Molecular Weight:70898
- Storage Temperature:At −20˚C for one year. After reconstitution, at 4 ˚C for one month. It can also be aliquotted and stored frozen at −20 ˚C for a longer time. Avoid repeated freezing and thawing.
- Concentration:Add 0.2 ml of distilled water will yield a concentration of 500 μg/ml
- Shipping Temperature:4 °C
- Immunogen:E.coli-derived human Hsc70 recombinant protein (Position: Q520-A614). Human Hsc70 shares 98.9% amino acid (aa) sequence identity with both mouse and rat Hsc70.
- Purification:Immunogen affinity purified
- Cat. No.:76174-138
Specifications
About this item
Rabbit IgG polyclonal antibody for Heat shock cognate 71 kDa protein(HSPA8) detection. Tested with WB, IHC-P in Human;Mouse;Rat.
HSPA8 (heat shock 70kDa protein 8) also known as HSC70, HSC71, HSP73, HSPA10, FORMERLY, LAP1 or LPS-ASSOCIATED PROTEIN 1, is a heat shock protein that in humans is encoded by the HSPA8 gene. The HSPA8 gene contains 9 exons and spans 5 kb. The deduced HSPA8 protein has 646 amino acids and a predicted molecular mass of 70,899 Da. And the HSPA8 gene is mapped on 11q24.1. HSPA8 plays an important role in cells by transiently associating with nascent polypeptides to facilitate correct folding. HSP73 also functions as an ATPase in the disassembly of clathrin-coated vesicles during transport of membrane components through the cell. Rapid decay involves AU-rich binding protein AUF1, which complexes with heat-shock proteins HSC70 and HSP70, translation initiation factor EIF4G, and poly (A)-binding protein. In the absence of Il3, Hsc70 formed a complex with Hsp40 and Hip, and this complex, in association with Eif4g and Pabp, formed a high-stability complex with Bim mRNA that protected it from ribonucleases.
Add 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.
Tested Species: In-house tested species with positive results.
By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.
Other applications have not been tested.
Optimal dilutions should be determined by end users.
Type: Primary
Antigen: HSPA8
Clonality: Polyclonal
Clone:
Conjugation:
Epitope:
Host: IgG
Isotype: Rabbit
Reactivity: Human; Mouse; Rat