Specifications
- Antibody Type:Primary
- Antigen Name:Histidine triad nucleotide binding protein 1
- Antigen Symbol:HINT1
- Clonality:Polyclonal
- Host:Rabbit
- ImmunoChemistry:Yes
- Isotype:IgG
- Reactivity:Human,Rat,Mouse
- Western Blot:Yes
- Size:100 µg/vial
- Form:Lyophilized
- Gene ID:P49773
- Antigen Synonyms:NMAN|PRKCNH1|PKCI-1|HINT
- UniProtKB:P49773
- Molecular Weight:13802
- Storage Temperature:At −20˚C for one year. After reconstitution, at 4 ˚C for one month. It can also be aliquotted and stored frozen at −20 ˚C for a longer time. Avoid repeated freezing and thawing.
- Concentration:Add 0.2 ml of distilled water will yield a concentration of 500 μg/ml
- Shipping Temperature:4 °C
- Immunogen:A synthetic peptide corresponding to a sequence in the middle region of human HINT1 (59-92aa HISQISVAEDDDESLLGHLMIVGKKCAADLGLNK), different from the related mouse and rat sequences by three amino acids.
- Purification:Immunogen affinity purified
- Cat. No.:76174-120
Specifications
About this item
Rabbit IgG polyclonal antibody for Histidine triad nucleotide-binding protein 1(HINT1) detection. Tested with WB, IHC-P in Human;Mouse;Rat.
Histidine triad nucleotide-binding protein 1 is a protein that in humans is encoded by the HINT1 gene. It is a haploinsufficient tumor suppressor gene that inhibits the Wnt/?-catenin pathway in colon cancer cells and Microphthalmia-associated transcription factor (MITF) activity in human mast cells. The protein encoded by this gene can hydrolyze substrates such as AMP-morpholidate, AMP-N-alanine methyl ester, AMP-alpha-acetyl lysine methyl ester, and AMP-NH2. The encoded protein interacts with these substrates via a histidine triad motif, which is part of the loop that binds to the substrate. Several transcript variants, but only one of them protein-coding, have been found for this gene.
Add 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.
Tested Species: In-house tested species with positive results.
By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.
Other applications have not been tested.
Optimal dilutions should be determined by end users.
Type: Primary
Antigen: HINT1
Clonality: Polyclonal
Clone:
Conjugation:
Epitope:
Host: IgG
Isotype: Rabbit
Reactivity: Human; Mouse; Rat