- Antibody Type:Primary
- Antigen Symbol:PPP2CA
- Clonality:Polyclonal
- Host:Rabbit
- ImmunoChemistry:Yes
- Isotype:IgG
- Reactivity:Human,Rat,Mouse
- Western Blot:Yes
- Size:100 µg/vial
- Form:Lyophilized
- Gene ID:P67775
- Molecular Weight:35594
- Storage Temperature:At −20 ˚C for one year. After reconstitution, at 4 ˚C for one month. It can also be aliquotted and stored frozen at −20 ˚C for a longer time. Avoid repeated freezing and thawing.
- Concentration:Add 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
- Shipping Temperature:4 °C
- Immunogen:E.coli-derived human PP2A-alpha recombinant protein (Position: M1-L309). Human PP2A-alpha shares 100% amino acid (aa) sequence identity with both mouse and rat PP2A-alpha.
- Purification:Immunogen affinity purified.
- Cat. No.:76173-528
Rabbit IgG polyclonal antibody for Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform(PPP2CA) detection. Tested with WB, IHC-P in Human;Mouse;Rat.
The catalytic subunit of human protein phosphatase 2A (PPP2CA) encodes a 309-amino acid polypeptide. It is localized to chromosome 5. The gene (approximately 30 kbp) is composed of seven exons and six introns. It is predicted to be important for phosphatase enzymatic activity. Methylation of the C-terminal leucine residue (Leu309) of protein serine/threonine phosphatase 2A catalytic subunit (PP2AC) is known to regulate catalytic activity in vitro. Furthermore, PP2A has a fundamental role in cardiac function, and suggests that disturbances in protein phosphatase expression and activity may cause or exacerbate the course of cardiac diseases.
Add 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Each vial contains 5 mg BSA, 0.9mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.
WB: The detection limit for PP2A-alpha is approximately 0.1ng/lane under reducing conditions.
Tested Species: In-house tested species with positive results.
By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.
Other applications have not been tested.
Optimal dilutions should be determined by end users.
Type: Primary
Antigen: PRDX1
Clonality: Polyclonal
Clone:
Conjugation:
Epitope:
Host: Rabbit
Isotype: IgG
Reactivity: Human, Mouse, Rat