Specifications
- Antibody Type:Primary
- Antigen Name:calpain 1, (mu/I) large subunit
- Antigen Symbol:CAPN1
- Clonality:Polyclonal
- Host:Rabbit
- ImmunoChemistry:Yes
- Isotype:IgG
- Reactivity:Human,Rat,Mouse
- Western Blot:Yes
- Size:100 µg
- Form:Lyophilized
- Gene ID:823
- Antigen Synonyms:muCANP|CANP|CANPL1|CANP1|muCL
- UniProtKB:P07384
- Molecular Weight:81890
- Storage Temperature:At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.
- Concentration:Add 0.2ml of distilled water will yield a concentration of 500 μg/ml.
- Shipping Temperature:4°C
- Immunogen:E.coli-derived human Calpain 1 recombinant protein (Position: Q396-A555). Human Calpain 1 shares 86% amino acid (aa) sequence identity with both mouse and rat Calpain 1.
- Purification:Immunogen affinity purified.
- Cat. No.:76173-494
- Packaging:Vial
Specifications
About this item
Rabbit IgG polyclonal antibody for Calpain-1 catalytic subunit(CAPN1) detection. Tested with WB, IHC-P in Human;Mouse;Rat.
CAPN1 is also known as CANP or muCL. The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 1. Several transcript variants encoding two different isoforms have been found for this gene.
Add 0.2 ml of distilled water will yield a concentration of 500µg/ ml.
Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO₄, 0.05 mg NaN₃.
WB: The detection limit for Calpain 1 is approximately 0.1 ng/lane under reducing conditions.
Tested Species: In-house tested species with positive results.
By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.
Other applications have not been tested.
Optimal dilutions should be determined by end users.
Type: Primary
Antigen: CASP8
Clonality: Polyclonal
Clone:
Conjugation:
Epitope:
Host: Rabbit
Isotype: IgG
Reactivity: Mouse