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Specifications
- Description:Mem-PER™ Extraction Kit
- Cat. No.:PI89826
- Application:Membrane Proteins from Cultured Mammalian and Yeast Cells, and Mammalian Tissues
- Supplier no.:89826
- Sufficient for:50−100 samples each containing 5 million cells, 15 mg yeast cell paste or 20 mg tissue
Specifications
About this item
The Mem-PER™ Eukaryotic Membrane Protein Extraction Kit enables small-scale solubilization and enrichment of hydrophobic membrane-associated proteins in a simple reagent-based procedure
- Extraction and isolation - produces minimal cross-contamination (typically less than 10%) of hydrophilic protein into the hydrophobic (membrane) protein fraction
- Cells or tissues - effect for extraction from cultured mammalian and yeast cells and mammalian tissues
- Downstream compatibility - analyze membrane protein extracts by SDS-PAGE, western blotting, and protein assays
- Fast and simple - isolation membrane proteins in approximately one hour
This kit effectively isolates membrane proteins from cultured cells and tissues using a simple benchtop microcentrifuge procedure. Hydrophobic proteins having three or fewer membrane-spanning domains are effectively solubilized and isolated from hydrophilic (cytoplasmic) proteins using the three-reagent system.