This buffer is intended for heat-induced antigen retriever on formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies.
In IHC most commonly used fixative like formalin mask tissue antigens (cellular, membrane and nuclear) by cross-linking process, this results in poor or no staining in IHC
Preparation of Working Solution: Dilute this 10X buffer as needed (e.g. 90 mL of deionized or distilled water+10 mL of this buffer) mix well, 1X buffer can be stored at 2 to 8 °C.
The use of this buffer on FFPE tissue section improves accessibility of antibodies to tissue antigens.
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