About this item
This buffer is intended for heat-induced antigen retriever on formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies.
- 1X buffer solution intended for heat-induced antigen retriever in IHC
- Use of this buffer on FFPE tissue section improves accessibility of antibodies to tissue antigens
- Please refer to primary antibody protocol
- Boiling point: 100 °C
- Density: 0.9989 g/cm³ (8.336 lbs/gal.)
In IHC most commonly used fixative like formalin mask tissue antigens (cellular, membrane and nuclear) by cross-linking process, this results in poor or no staining in IHC.
Preparation of working solution: dilute this 10X buffer as needed (e.g. 90 ml of deionized or distilled water+10 ml of this buffer) mix well, 1X buffer can be stored at 2 to 8 °C.
The use of this buffer on FFPE tissue section improves accessibility of antibodies to tissue antigens.
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