About this item
Trypsin is used for proteolytic digestion of formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies.
- Use as antigen retriever for some antibodies in IHC
- Please refer to primary antibody protocol for IHC
- Supplied as concentrated form along with buffer
In IHC most commonly used fixative like formalin; mask tissue antigens (cellular, membrane and nuclear) by cross-linking process, this results in poor or no staining in IHC. Trypsin digestion improves immunoreactivity of some antigens in FFPE tissue sections. For cytokeratin clone AE3 and AE1/AE3, this enzyme works much better than boiling the tissue with citrate buffer.
Large kit contains (100 mL) Reagent B Buffer and (10 mL) Reagent E (Enzyme).
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