About this item
HisDetector™ Nickel conjugates provides direct detection of recombinant proteins
- Fast, sensitive, single-step method for detecting recombinant His-tagged proteins
- Results in assay simplification and reduced detection times
- Pptimized for use with both chemiluminescent and colorimetric substrates
Also they offers excellent sensitivity and low background in applications such as western blotting and may also be used in other application (ELISA, immunohistochemistry, etc.) where direct detection of His-tagged proteins is required.
His-tagged proteins have a high affinity to nickel (Ni2+) ions. Nickel-NTA (nitrilotriacetic acid) conjugated to horseradish peroxidase (HRP) and alkaline phosphatase(AP) will therefore bind directly to the His-tag, eliminating the need for primary and secondary antibodies.
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