Prepare for sequencing success with the highest quality library.

• Simplified 2-step protocol speeds sample prep to under 3 hours and minimizes sample loss from transfer steps
• Increased library yields enable the construction of low input DNA sample from 250 pg
• Minimized bias improves coverage across difficult to sequence regions ensuring optimal results and reduced coverage gaps
• PCR-free workflows enabled from 100 ng input DNA
• Improved overall sequencing workflow economics

An optimized kit for the rapid construction of DNA libraries from fragmented double-stranded DNA for sequencing on Illumina® NGS platforms

Maximize library yields and increase sequenceable molecules
Critical first steps in library preparation depends heavily on the efficiency and sensitivity of the enzymes involved in DNA polishing and adapter ligation steps. The sparQ enzymes have been engineered, optimized, and selected for superior performance. The proprietary cocktail of enzymes is formulated to generate the highest yield and quality of adapter-ligated libraries over a broad range of input DNA down to as little as 250 pg enabling successful library construction for challenging samples and PCR-free workflows where input DNA is ≥100 ng.

Minimize bias and gaps in sample genome coverage
The highly efficient library prep reduces the bias resulting in the superior quality you need and expect, to minimize coverage gaps especially for challenging regions like GC- and AT-rich sequences. For applications requiring amplification, the high fidelity master mix is formulated to increase library yield reducing the number of cycles required to create a sequence-ready library thereby reducing additional PCR-derived artifacts.

Create amplified libraries with PCR-free results
Comparison of library preparation performed with sparQ DNA Library Prep Kit matches PCR-free workflows. Low amplification bias enables better coverage uniformity resulting in greater sequencing depth or multiplexing capabilities.

DNA polishing reactions are combined in a single step to convert fragmented DNA into 5'-phosphorylated and 3'-dA-tailed DNA fragments suitable for direct ligation of sequencing adapters without the need for an intervening cleanup saving valuable time and bead purification expense. The HiFi PCR Master Mix and Primer Mix allows for the optional, unbiased amplification of fragments with appropriate adapters ligated to both ends. The kit is compatible with input amounts from 250 pg to 1 μg DNA and multiple sample types.