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- Conjugation:Unconjugated
- Protein/Peptide Type:Recombinant
- Source:E. coli
- Species:Human
- Size:10 µg
- Tag sequence:GST Tag
- Storage Conditions:Store at 2-4 °C for immediate use within 1-2 weeks or at –80 °C for up to 12 months. Avoid repeated freeze-thaw cycles.
- Gene ID:NP_001116849
- Protein Synonyms:PARK7 GST|PARK7|DJ - 1|DJ-1 GST|Human DJ-1|Human PARK7
- Protein/Peptide Name:DJ-4
- Purity:> 95% as determined by SDS-PAGE
- Molecular Weight:19.9 kDa
- Cat. No.:103010-652
- Supplier no.:AS-72220-10
The sequence (Accession # NP_001116849) corresponding to the full length human DJ-1 protein along with N-terminal GST tag was expressed in E. coli. The recombinant DJ-1 protein was purified from bacterial lysate using GST affinity chromatography. GST tag was not removed. The molecular weight of the recombinant GST-DJ-1protein is 47.3 kDa.
DJ-1 is also known as PARK7 (Parkinson disease protein 7). It is a 189-amino acid protein that is ubiquitously expressed in many organs including brain, liver, kidney, pancreas, heart, and others. Although DJ-1 was originally discovered as a novel oncogene product, it was found to play several other roles in biological processes. This includes the regulation of RNA binding activity, fertility, anti-oxidative stress, and is linked to the early onset of Parkinson disease when mutated. Sequence (Accession# NP_001116849) corresponds to the full length human DJ-1 protein and was expressed in E. coli.