- Antibody Type:Primary
- Antigen Name:CD80 molecule
- Antigen Symbol:CD80
- Clonality:Monoclonal
- Clone:10A1
- Conjugation:Unconjugated
- ELISA:Yes
- Flow Cytometry:Yes
- Host:Mouse
- ImmunoChemistry:Yes
- ImmunoFluorescence:Yes
- Isotype:IgG2b
- Reactivity:Human
- Size:0.1 mg
- Format:Purified Antibody
- Form:Liquid
- Gene ID:P33681
- Antigen Synonyms:CD80
- Storage Buffer:CD80 Antibody is supplied in PBS containing 0.02% sodium azide.
- Molecular Weight:Predicted: 32 kDa Observed: 50 kDa
- Storage Temperature:CD80 antibody can be stored at 4 °C for three months and –20 °C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
- Concentration:1 mg/mL
- Shipping Temperature:4 °C
- Immunogen:CD80 antibody was raised against the extracellular domain of human CD80.
- Tested Applications:Immunocytochemistry
- Purification:CD80 Antibody is supplied as protein A purified IgG2b.
- Cat. No.:76163-578
CD80 Antibody: CD80, also known as B7-1, is a type I membrane protein that is a member of the immunoglobulin superfamily. Like the related protein CD86, this protein is expressed by antigen-presenting cells, and is the ligand for two proteins at the cell surface of T cells, CD28 and the cytotoxic T-lymphocyte-associated protein 4 (CTLA-4). Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell and induces T-cell proliferation and cytokine production. CTLA-4 binding negatively regulates T-cell activation and diminishes the immune response (1). Blocking the CTLA-4-CD80/CD86 interaction has been shown to enhance T-cell functions in acute lymphoblastomic leukemia (ALL), suggesting that this pathway may be an attractive target for future cancer immunotherapy (2).
CD80 antibody can be used for immunohistochemistry starting at 2 - 5 g/mL. For immunofluorescence start at 5 g/mL. Flow cytometry at 10 g/ml.
Type: Primary
Antigen: CD80
Clonality: Monoclonal
Clone: 10A1
Conjugation: Unconjugated
Epitope:
Host: Mouse
Isotype: IgG2b
Reactivity: Human