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- Description:SensoLyte® 440 Cathepsin B Assay Kit *Fluorimetric*
- Size:1 Kit
- Storage Temperature:-20°C
- Cat. No.:103010-534
- No. of wells:96
- No. of assays/kit:100 assays
- Supplier no.:AS-72165
Cathepsin B is a member
Cathepsins are a class of globular lysosomal proteases playing a vital role in mammalian cellular turnover. They degrade polypeptides and are distinguished by their substrate specificities.
The cathepsin assay kits offers a series of fluorimetric long wavelength, sensitive SensoLyte® 520 assay kits for enzyme activity determination of Cathepsins G, D, E, B, K, L and S. These kits employ a FRET (Fluorescence Resonance Energy Transfer) peptide substrate that emits in the green range of 520 nm, with excitation at 490 nm. The advantage of using longer wavelength emitting substrates means that autofluorescence from cellular components and test compounds is minimal; thus making the kits more sensitive. Furthermore, the use of HiLyte Fluor™ 488 (except for Cathepsin S, which uses 5-FAM) as the FRET donor (fluorophore) results in a more stable fluorescent signal, since HiLyte Fluor™ 488 is resistant to acidic pH. A listing of the SensoLyte® Cathepsin Kits providing conventional FRET (Mca/Dnp) or fluorogenic substrates for cathepsins are also available.
Kits detect specific Cathepsin activities; provides ample reagents to perform 100 assays in 96-well format; minimal hands-on time required; detailed protocol and references provided.
Elevated levels of cathepsin B were detected in metastases and neurological disorders including Alzheimer's disease (AD). The SensoLyte® 440 Cathepsin B Assay Kit provides a fluorogenic peptide for measurement of enzyme activity. This peptide releases the AMC (7-amino-4-methylcoumarin) fluorophore upon cathepsin B cleavage and can be detected with excitation at 354 nm and emission at 442 nm.