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Specifications
- Description:SensoLyte® pNPP Secreted Alkaline Phosphatase Reporter Gene Assay Kit *Colorimetric*
- Size:1 Kit
- Storage Temperature:-20°C
- Cat. No.:103010-490
- No. of wells:96
- No. of assays/kit:500 assays
- Supplier no.:AS-72144
Specifications
About this item
The secreted alkaline phosphatase (SEAP) is widely used as reporter gene to analyze gene expression including kinetic analysis over time in cell culture or animals owing to its unique ability to secrete into culture medium and serum
- Features: Kits include all essential components (chromogenic/fluorogenic/luminometry substrates) and sample reagents needed to perform 500 assays using 96-well plate format.
- Optimized to detect specific alkaline phosphatase activity; compatible for HTS; detailed protocol and references provided.
Phosphatases are important classes of enzymes involved in cellular signal transduction, cell metabolism and other biological activities. Changes in alkaline phosphatase level and activity are involved in a variety of physiological and pathological events, such as bone development, bone-related diseases, gestation related diseases, inflammatory bowel disease, post-parathyroidectomy stage and drug toxicity. Phosphatases have attracted considerable interest as: Enzyme markers, Drug screening targets, Conjugating agents and Reporter for gene expression studies.
SensoLyte® pNPP Secreted Alkaline Phosphatase Reporter Gene Assay Kit provides a sensitive colorimetric assay of placental alkaline phosphatase for both secreted and membrane-bound form of enzyme