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- Application:
- Method / Format:Precipitation
- Prep size:Mini
- Refine target molecule:Yeast/Fungal DNA
- Sample type:Yeast/Fungi
- Target molecule:DNA
- Description:MasterPure™ Yeast DNA Purification Kit, Lucigen / Epicentre
- Size:200 Purifications
- Environmentally Preferable:
- Description:MasterPure™ Yeast DNA Purification Kit
- Cat. No.:76081-694
Purify high yields of quality genomic DNA from yeast with this easy to use kit
The MasterPure™ Yeast DNA Purification Kit enables efficient, high-yield purification of high-molecular-weight DNA from yeast and other fungi. The protocol involves nonenzymatic cell lysis at 65°C, followed by removal of protein by precipitation, and nucleic acid precipitation and resuspension. No lyticase, proteolytic enzymes, or bead-beating are used in the procedure. Yeast genomic DNA yields using the MasterPure Kit are much higher than yields obtained with other commercially available kits. The protocol can be easily adjusted for larger or smaller samples, including single yeast colonies. The recovered nucleic acid can be used directly in most applications, including PCR amplification.
Fast: Obtain purified DNA in less than 40 minutes
Flexible: Purify DNA from a wide variety of yeast species, including Candida, Saccharomyces, Pichia, and Schizosaccharomyces, and filamentous fungi such as Aspergillus and Penicillium
High Quality DNA: Purify high molecular weight genomic DNA that is ready to use in many molecular biology applications, including PCR amplification, restriction endonuclease digestion, Southern blotting, genomic library preparation and fungal identification and typing
High Yields: Purify more yeast genomic DNA than other commercially available kits
Simple: No enzymatic lysis, bead beating, columns, phenol or other organic solvents are used