RNase R

A 3´ to 5´ exoribonuclease that digests all linear RNAs except lariat or circular RNA structures making it well-suited to RNA splicing and other research applications

• Specific: Digests all linear RNAs but does not digest lariat or circular RNA structures, or doublestranded RNA with 3' overhangs shorter than seven nucleotides
• Heat Inactivated: Heat at 65°C for 20 minutes to kill enzyme activity
• Valuable: Use the unique properties of this exoribonuclease to study alternative splicing and gene expression

Ribonuclease R (RNase R) from E

Lariat RNAs isolated using this method can be used as a template to produce labeled cDNA as a target for microarrays containing potential intron sequences, or for tiling arrays containing overlapping regions of complete chromosomes or genomes. The cDNA produced will not be a linear representation of the intron, but the sequences contained in it will be intron-derived.

Applications include:
Alternative splicing and gene expression studies
Intron cDNA production
Intronic screening of cDNA libraries

coli is a magnesium-dependent 3´ to 5´ exoribonuclease that digests essentially all linear RNAs but does not digest lariat or circular RNA structures, or doublestranded RNA with 3´ overhangs shorter than seven nucleotides. Most cellular RNAs will be digested completely by RNase R, with the exception of tRNAs, 5S RNA, and intron lariats. The 3´ tails of lariats will be trimmed by RNase R to the branch point nucleotide, where there is a 2´,5´-phosphodiester linkage. Lariats are produced during pre-mRNA splicing of intron regions and can be isolated from a mixture of total RNA by digestion with RNase R.

Certifications: For research use only. Not for human or diagnostic use.

Ordering information: Contents include enzyme and a 10X reaction buffer.