Magnesium-dependent digestion of single-stranded DNA in a 5´ to 3´ direction

• Specific: Digests ssDNA in the 5' to 3 direction
• Heat Inactivated: Heat at 65°C for 20 minutes to kill enzyme activity
• Flexible: Use to remove primers from completed PCR or to degrade single-stranded linear DNA in dsDNA and plasmid preps

Rec J Exonuclease, derived from E

Applications include:
Removal of primers from completed PCR
Degradation of single-stranded linear DNA in dsDNA and plasmid preps

coli, catalyzes degradation of ssDNA in a 5´ to 3´ direction. Its activity is dependent on Mg2+. Rec J Exonuclease can be heat-inactivated by incubation at 65°C for 20 minutes.