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Specifications
- Application:
- Description:Exo-Minus Klenow DNA Polymerase (D355A, E357A)
- Size:1,000 units
- Environmentally Preferable:
- Concentration:10 units/μL
- Cat. No.:76081-728
Specifications
About this item
An improved Klenow DNA polymerase lacking all 5' to 3' and 3' to 5' exonuclease activity
- Synthesize DNA from ssDNA templates without degrading any single-stranded DNA
- Suitable for a variety of applications including random-primer labeling of DNA, second-strand cDNA synthesis, strand-displacement amplification
Exo-Minus Klenow DNA Polymerase is a DNA-dependent DNA polymerase that lacks both the 5´ to 3´ and 3´ to 5´ exonuclease activities of E. coli DNA Polymerase I, from which it is derived. This N-terminal truncation of DNA Polymerase I also has two mutations (D355A and E357A).