10X Tris-buffered Saline (TBS) with Tween 20 is used as a common western blot wash solution and general wash buffer in immunoassay applications

Formulation of TBST with higher concentration of Tween 20 provides a more stringent wash than standard TBST formulations.

Tween 20, a non-ionic detergent, reduces non-specific binding and protein-protein interaction during the wash step in immunoassay procedures.