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Specifications
- Description:PolyHis-Protein:Protein Interaction Kit
- Cat. No.:PI21277
- No. of reactions:25 Pull-down reactions, each using 25 µl of resin
- Supplier no.:21277
Specifications
About this item
The Pierce™ His Protein Interaction Pull-Down Kit contains the necessary components to capture and purify proteins that interact with His-tagged fusion proteins.
- Complete kits provide all components and detailed protocols for purifying protein:protein interactions
- No special equipment needed – use common laboratory equipment and reagents
- Microcentrifuge spin columns facilitate simple and efficient manipulation of agarose beads, including simple processing of multiple samples
- Instructions include protocols for use with bait and prey proteins expressed from a variety of sample types
- Cobalt chelate resin is more specific for histidine-tagged fusion proteins than nickel resins, resulting in less non-specific binding
- Binds 10 to 25 mg of histidine-tagged fusion protein per mL of resin
Applications include discover a new protein:protein interaction from a cell lysate; confirm a putative interaction from a cell lysate or with a previously purified protein; extract protein:protein interaction information from in vitro transcription/translation lysates.