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- Description:Phosphoprotein Phosphate Estimation Assay Kit
- Cat. No.:PI23270
- No. of assays/kit:500 tube assays or 1920 microplate assays
- Supplier no.:23270
Pierce™ Phosphoprotein Phosphate Estimation Assay Kit enables classification and identification of proteins as phosphorylated (serine and threonine), as well as semi-quantitative assessment of the phosphorylation level.
- Specifically measures phosphoserine (p-Ser) and phosphothreonine (p-Thr) only
- Test tube and 96-well microplate protocols included and require less than 90 minutes to perform
- Semi-quantitative, calculate moles of phosphate per mole of protein using the included phosvitin standard
- Format is adaptable to development of specific assays for pure phosphoproteins when previously characterized standards are available
Applications include identification of proteins as containing phosphoserine or phosphothreonine phosphorylations; estimating the amount of pS and pT phosphorylation; developing specific quantitative assays for well-characterised phosphoproteins of interest.