Alternative E.coli strains for bacterial transformation and lysis.

• Straightforward transformation procedure with up to 10^8 - 10^9 ^{transformants}/_μg plasmid.
• Simple, fast, and controlled autolysis of E. coli.
• Available with DE3 lysogen for T7 promoter transcription.

XJ Autolysis™ E. coli strains are a new alternative for bacterial transformation and lysis. These strains are efficiently lysed following arabinose-induced expression of the bacteriophage λ endolysin protein, coupled to a single freeze-thaw cycle. The strains simplify protein expression and purification. They are also applicable for nucleic acid purification, and available with a DE3 lysogen encoding the T7 polymerase for expressing recombinant proteins driven by the T7 promoter.