Mouse Recombinant TNF-alpha (from HEK293 cells)

Tumor necrosis factor (TNF, cachexin or cachectin and formerly known as tumor necrosis factor-alpha) is a cytokine involved in systemic inflammation and is a member of a group of cytokines that stimulate the acute phase reaction. MultimericTNF-alpha™ is a high activity construct in which two trimeric TNF-alpha ligands are artificially linked via the collagen domain of ACRP30. The receptor TNF-R1 is activated by both the membrane-bound and soluble trimeric forms of TNF-alpha, whereas the receptor TNF-R2 only responds to the membrane-bound form of TNF-alpha (TNF-alpha (mouse) (multimeric) (rec.) (Prod. No. AG-40B-0021). Since the MultimericTNF-alpha™ mimics the membrane-bound form (forms oligomers higher than trimer), it is the only TNF-alpha protein that can activate the TNF-R2. For TNF-R1 activation, either "normal" TNF-alpha or MultimericTNF-alpha™ can be used.

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Conjugation:
Unconjugated
Protein/Peptide Type:
Recombinant
Source:
HEK293 cells
Species:
Mouse
Storage Conditions:
–20 °C
Endotoxin Content:
<0.02EU/µg
Biological Activity:
Activates human and mouse TNF-R1 and TNF-R2. Induces cell death at a concentration range of 0.01-10ng/ml (ED50= 0.05ng/ml or 2 x 10E7 Units/mg; WEHI 164 cells).
Gene ID:
P06804
Protein Synonyms:
ACRP30headless:TNF-alpha|ACRP30headless:Tumor Necrosis Factor-alpha|ACRP30headless:TNFSF2|MultimericTNF-alpha™
Protein/Peptide Name:
TNF-alpha
Purity:
95%
Molecular Weight:
~34kDa (SDS-PAGE)
Endotoxin Level:
Low
Concentration:
0.1mg/ml after reconstitution.
Formulation:
Lyophilized. Contains PBS.
Shipping Temperature:
-20 °C, Blue Ice