About this item
Tumor necrosis factor (TNF, cachexin or cachectin and formerly known as tumor necrosis factor-alpha) is a cytokine involved in systemic inflammation and is a member of a group of cytokines that stimulate the acute phase reaction. MultimericTNF-alpha™ is a high activity construct in which two trimeric TNF-alpha ligands are artificially linked via the collagen domain of ACRP30. The receptor TNF-R1 is activated by both the membrane-bound and soluble trimeric forms of TNF-alpha, whereas the receptor TNF-R2 only responds to the membrane-bound form of TNF-alpha (TNF-alpha (mouse) (multimeric) (rec.) (Prod. No. AG-40B-0021). Since the MultimericTNF-alpha™ mimics the membrane-bound form (forms oligomers higher than trimer), it is the only TNF-alpha protein that can activate the TNF-R2. For TNF-R1 activation, either "normal" TNF-alpha or MultimericTNF-alpha™ can be used.
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Specifications
- Conjugation:Unconjugated
- Protein/Peptide Type:Recombinant
- Source:HEK293 cells
- Species:Mouse
- Storage Conditions:–20 °C
- Endotoxin Content:<0.02EU/µg
- Biological Activity:Activates human and mouse TNF-R1 and TNF-R2. Induces cell death at a concentration range of 0.01-10ng/ml (ED50= 0.05ng/ml or 2 x 10E7 Units/mg; WEHI 164 cells).
- Gene ID:P06804
- Protein Synonyms:ACRP30headless:TNF-alpha|ACRP30headless:Tumor Necrosis Factor-alpha|ACRP30headless:TNFSF2|MultimericTNF-alpha™
- Protein/Peptide Name:TNF-alpha
- Purity:95%
- Molecular Weight:~34kDa (SDS-PAGE)
- Endotoxin Level:Low
- Concentration:0.1mg/ml after reconstitution.
- Formulation:Lyophilized. Contains PBS.
- Shipping Temperature:-20 °C, Blue Ice