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Specifications
- Antibody Type:Primary
- Antigen Symbol:HMGB1
- Clone:Giby-1-4
- Conjugation:Biotin
- ELISA:Yes
- Host:HEK293
- Isotype:IgG2 lambda
- Reactivity:Human,Rat,Mouse
- Western Blot:Yes
- Size:100 µg
- Form:liquid
- Gene ID:P09429
- Antigen Synonyms:High Mobility Group Protein B1
- Storage Temperature:–20 °C
- Concentration:1mg/ml
- Shipping Temperature:-20° C
- Immunogen:Human recombinant HMGB1.
- Cat. No.:102979-998
- Supplier no.:AG27B0002BC100
Specifications
About this item
HMGB1 was originally discovered as an essential DNA-binding protein for regulating p53, NF-kappaB and other important proteins. It is secreted from activated dentric cells, macrophage and nectrotic cells, and acts as a ligand for RAGE, TLR-2 and TLR-4 expressed on surrounding cells. As a result, HMGB1 activates Rac, CDC42 and NF-kappaB inducing localized innate immunity of damaged tissue, tissue regeneration by recruitment of stem cells and hemostasis by induction of tissue factor expression. HMGB1 is also a causative agent of various diseases as it causes localized inflammation such as arteriosclerosis, chronic rheumatoid arthritis and nephritis. Anti-HMGB1, mAb (recombinant) (Giby-1-4) (Biotin) is an antibody developed by antibody phage display technology using a human naive antibody gene library. These libraries consist of scFv (single chain fragment variable) composed of VH (variable domain of the human immunoglobulin heavy chain) and VL (variable domain of the human immunoglobulin light chain) connected by a polypeptide linker. The antibody fragments are displayed on the surface of filamentous bacteriophage (M13). This scFv was selected by affinity selection on antigen in a process termed panning. Multiple rounds of panning are performed to enrich for antigen-specific scFv-phage. Monoclonal antibodies are subsequently identified by screening after each round of selection. The selected monoclonal scFv is cloned into an appropriate vector containing a Fc portion of interest and then produced in mammalian cells to generate an IgG like scFv-Fc fusion protein.
Type: Primary
Antigen: HMGB1
Clonality: Recombinant
Clone: Giby-1-4
Conjugation: Biotin
Epitope:
Host: Purified from HEK 293 cell culture supernatant.
Isotype: Human IgG2lambda
Reactivity: Human, Mouse, Rat