- Antibody Type:Primary
- Antigen Name:Cell cycle checkpoint protein RAD17
- Antigen Symbol:RAD17
- Clonality:Polyclonal
- Conjugation:Unconjugated
- Flow Cytometry:Yes
- Host:Rabbit
- Reactivity:Human
- Western Blot:Yes
- Size:400 ul
- Epitope:Amino Acid Range = 218-246
- Form:Liquid
- Gene ID:O75943
- Antigen Synonyms:RAD17
- Modification:None
- Storage Buffer:Supplied in PBS with 0.09% (W/V) sodium azide.
- Molecular Weight:77 kDa
- Storage Temperature:Store at 4 °C for three months and –20 °C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
- Concentration:0.46 mg/mL
- Shipping Temperature:4 °C
- Immunogen:This RAD17 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 218-246 amino acids from the Central region of human RAD17.
- Purification:This antibody is purified through a protein A column, followed by peptide affinity purification.
- Cat. No.:76011-048
The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad17, a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage. This protein shares strong similarity with DNA replication factor C (RFC), and can form a complex with RFCs. This protein binds to chromatin prior to DNA damage and is phosphorylated by the checkpoint kinase ATR following damage. This protein recruits the RAD1-RAD9-HUS1 checkpoint protein complex onto chromatin after DNA damage, which may be required for its phosphorylation. The phosphorylation of this protein is required for the DNA-damage-induced cell cycle G2 arrest, and is thought to be a critical early event during checkpoint signaling in DNA-damaged cells. Eight alternatively spliced transcript variants of this gene, which encode four distinct proteins, have been reported. Two pseudogenes, located on chromosomes 7 and 13, have been identified.
For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50
Type: Primary
Antigen: RAD17
Clonality: Polyclonal
Clone:
Conjugation: Unconjugated
Epitope:
Host: Rabbit
Isotype: Rabbit Ig
Reactivity: Human