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- Description:SDS-PAGE Running Buffer, 10X
- Size:1 L
- Environmentally Preferable:
- Cat. No.:RLMB-017
- pH:8.3
- Supplier no.:MB-017
SDS-PAGE, sodium dodecyl sulfate polyacrylamide gel electrophoresis, describes a technique widely used in biochemistry to separate proteins according to their electrophoretic mobility (a function of the length of a polypeptide chain and its charge) and no other physical feature
SDS is an anionic detergent applied to protein sample to linearize proteins and to impart a negative charge to linearized proteins
This product is a 10X concentrated stock solution and should be diluted appropriately with distilled, deionized water (or equivalent) to its final working concentration. No pH adjustment is required. 10X SDS-PAGE Running Buffer consists of 0.25 M Tris HCl, 1.92 M Glycine and 1% (w/v) Sodium Dodecyl Sulfate (SDS); pH 8.3. Meticulously prepared using ultra-pure reagents dissolved in highly polished pharmaceutical grade deionized water.