TransforMax™EC100™ Electrocompetent and Chemically Competent E. coli, Biosearch Technologies
About this item
Clone large plasmids with high efficiency.
- Clone large inserts and transform large plasmids - up to at least 145 kb plasmid DNA
- Select convenience with chemically competent format: >5 x 108 cfu/µg pUC19 DNA
- Choose electrocompetent format for higher efficiencies: >1 x 1010 cfu/µg pUC19 DNA
- Ideal strain for general cloning, subcloning and plasmid preparation due to endA and recA mutation
The highly versatile TransforMax™ EC100™ E
EC100 competent cells are recombination minus (recA) and endonuclease minus (endA), for high yield, high quality plasmid DNA preparations. They are also blue/white screening capable (lacZΔM15) and restriction minus [mcrA, Δ(mrr-hsdRMS-mcrBC)] for cloning methylated DNA.
Genotype: F- mcrA Δ(mrr-hsdRMS-mcrBC) Φ80dlacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara, leu)7697 galU galK λ- rpsL (StrR) nupG.
coli competent cells are ideal for most cloning applications. The cells provide very high transformation efficiency when tested against a wide range of supercoiled DNAs as well as DNA directly from a ligation reaction, including BAC clones.
: TransforMax™ EC100™ Chemically Competent E. coli kit contains ten tubes, each containing 50 µL of cells (enough cells for 10 transformations) and pUC19 control DNA.
TransforMax™ EC100™ Electrocompetent E. coli kit contains ten tubes, each containing 100 µL of cells (enough cells for 20 transformations) and pUC19 control DNA.
: For research use only. Not for human or diagnostic use.
2 Options Available Below
- Item requires temperature control for storage and delivery with additional fees. It's not eligible for return due to safety and quality concerns. Consider requirements before purchasing.
- Return Policy
Product Details & Documents
Clone large plasmids with high efficiency.
- Clone large inserts and transform large plasmids - up to at least 145 kb plasmid DNA
- Select convenience with chemically competent format: >5 x 108 cfu/µg pUC19 DNA
- Choose electrocompetent format for higher efficiencies: >1 x 1010 cfu/µg pUC19 DNA
- Ideal strain for general cloning, subcloning and plasmid preparation due to endA and recA mutation
The highly versatile TransforMax™ EC100™ E
EC100 competent cells are recombination minus (recA) and endonuclease minus (endA), for high yield, high quality plasmid DNA preparations. They are also blue/white screening capable (lacZΔM15) and restriction minus [mcrA, Δ(mrr-hsdRMS-mcrBC)] for cloning methylated DNA.
Genotype: F- mcrA Δ(mrr-hsdRMS-mcrBC) Φ80dlacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara, leu)7697 galU galK λ- rpsL (StrR) nupG.
coli competent cells are ideal for most cloning applications. The cells provide very high transformation efficiency when tested against a wide range of supercoiled DNAs as well as DNA directly from a ligation reaction, including BAC clones.
: TransforMax™ EC100™ Chemically Competent E. coli kit contains ten tubes, each containing 50 µL of cells (enough cells for 10 transformations) and pUC19 control DNA.
TransforMax™ EC100™ Electrocompetent E. coli kit contains ten tubes, each containing 100 µL of cells (enough cells for 20 transformations) and pUC19 control DNA.
: For research use only. Not for human or diagnostic use.
Product Family Options
Product Information
- SizeAvailabilityPrice
- 75927-880TransforMax™ EC100™ Chemically Competent E. coliSupplier Number: CC02810Item requires temperature control for storage and delivery with additional fees. It's not eligible for return due to safety and quality concerns. Consider requirements before purchasing.
- 75927-926TransforMax™ EC100™ Electrocompetent E. coliSupplier Number: EC10010Item requires temperature control for storage and delivery with additional fees. It's not eligible for return due to safety and quality concerns. Consider requirements before purchasing.
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